LEF1 (NM_016269) Human Recombinant Protein

• Background:

  This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

• Synonyms:

  LEF-1; TCF1ALPHA; TCF7L3; TCF10

• Gene ID:

  51176

• UniProt:

  Q9UJU2, Q659G9

• Accession Number mRNA:

  NM_016269

• Chromosomal Location:

  4q25

• Expression System:

  E. coli

• Tag:

  N-His

• Related Pathways:

  Acute myeloid leukemia, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Melanogenesis, Pathways in cancer, Prostate cancer, Thyroid cancer, Wnt signaling pathway

• Field of Research:

  Acute myeloid leukemia, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Melanogenesis, Pathways in cancer, Prostate cancer, Thyroid cancer, Wnt signaling pathway

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl

• Function:

  Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors

• Molecular Weight:

  44 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  LEF1

• Species:

  Human

• Protein ID:

  NP_057353

• Overview:

  Recombinant protein of human lymphoid enhancer-binding factor 1 (LEF1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/51176

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9UJU2

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_016269

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_057353