TFEB (NM_007162) Human Recombinant Protein

• Background:

  Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3') . Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4 (+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  ALPHATFEB; BHLHE35; TCFEB

• Gene ID:

  7942

• UniProt:

  P19484, A0A024RCY3

• Accession Number mRNA:

  NM_007162

• Chromosomal Location:

  6p21.1

• Expression System:

  E. coli

• Tag:

  N-His

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  27172265

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl.

• Function:

  Druggable Genome, Transcription Factors

• Molecular Weight:

  52.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  TFEB

• Species:

  Human

• Protein ID:

  NP_009093

• Overview:

  Recombinant protein of human transcription factor EB (TFEB), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/7942

• Uniprot URL:

  https://www.uniprot.org/uniprot/P19484

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_007162

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_009093