PDGF beta (PDGFB) (NM_002608) Human Recombinant Protein

• Background:

  This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF) . The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

• Synonyms:

  C-sis; IBGC5; PDGF-2; PDGF2; SIS; SSV

• Gene ID:

  5155

• UniProt:

  P01127, A0A384NYY3

• Accession Number mRNA:

  NM_002608

• Chromosomal Location:

  22q13.1

• Expression System:

  E. coli

• Tag:

  N-His

• Related Pathways:

  Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma

• Field of Research:

  Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl.

• Function:

  Druggable Genome

• Molecular Weight:

  24.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PDGFB

• Species:

  Human

• Protein ID:

  NP_002599

• Overview:

  Purified recombinant protein of Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/5155

• Uniprot URL:

  https://www.uniprot.org/uniprot/P01127

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_002608

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_002599