PRCP (NM_005040) Human Recombinant Protein

• Background:

  This gene encodes a member of the peptidase S28 family of serine exopeptidases. The encoded preproprotein is proteolytically processed to generate the mature lysosomal prolylcarboxypeptidase. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. A pseudogene of this gene has been identified on chromosome 2. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

• Synonyms:

  HUMPCP; PCP

• Gene ID:

  5547

• UniProt:

  P42785, A0A024R5L0

• Accession Number mRNA:

  NM_005040

• Chromosomal Location:

  11q14.1

• Expression System:

  E. coli

• Tag:

  N-His

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  50mM Tris, 8M Urea, pH8.0.

• Function:

  Druggable Genome, Protease, Transmembrane

• Molecular Weight:

  53.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PRCP

• Species:

  Human

• Protein ID:

  NP_005031

• Overview:

  Purified recombinant protein of Human prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 1, with N-terminal HIS tag, expressed in E.Coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/5547

• Uniprot URL:

  https://www.uniprot.org/uniprot/P42785

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_005040

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_005031