EEF1D (NM_032378) Human Recombinant Protein

• Background:

  This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010]

• Synonyms:

  EF-1D; EF1D; FP1047

• Gene ID:

  1936

• UniProt:

  P29692, B2RAR6, D3DWK1

• Accession Number mRNA:

  NM_032378

• Chromosomal Location:

  8q24.3

• Expression System:

  E. coli

• Tag:

  N-His

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1 % Sarkosyl.

• Molecular Weight:

  71.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  EEF1D

• Species:

  Human

• Protein ID:

  NP_115754

• Overview:

  Purified recombinant protein of Human eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (EEF1D), transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/1936

• Uniprot URL:

  https://www.uniprot.org/uniprot/P29692

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_032378

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_115754