DNase I (DNASE1) (NM_005223) Human Recombinant Protein

• Background:

  This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

• Synonyms:

  DNL1; DRNI

• Gene ID:

  1773

• UniProt:

  P24855

• Accession Number mRNA:

  NM_005223

• Chromosomal Location:

  16p13.3

• Expression System:

  E. coli

• Tag:

  N-GST and C-His

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1 % Sarkosyl.

• Function:

  Druggable Genome, Secreted Protein, Transmembrane

• Molecular Weight:

  55.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  DNASE1

• Species:

  Human

• Protein ID:

  NP_005214

• Overview:

  Purified recombinant protein of Human deoxyribonuclease I (DNASE1), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/1773

• Uniprot URL:

  https://www.uniprot.org/uniprot/P24855

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_005223

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_005214