SPDYA (NM_001142634) Human Recombinant Protein

• Background:

  Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2 (PubMed:12972555) . Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation (PubMed:28666995) . Mediates cell survival during the DNA damage process through activation of CDK2 (PubMed:12839962) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  RINGO3; RINGOA; SPDY1; SPY1

• Gene ID:

  245711

• UniProt:

  Q5MJ70, A0A384MTT5

• Accession Number mRNA:

  NM_001142634

• Chromosomal Location:

  2p23.2

• Expression System:

  E. coli

• Tag:

  N-His

• Related Pathways:

  Oocyte meiosis, Progesterone-mediated oocyte maturation

• Field of Research:

  Oocyte meiosis, Progesterone-mediated oocyte maturation

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  50mM Tris, 8M Urea, pH8.0.

• Molecular Weight:

  36.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SPDYA

• Species:

  Human

• Protein ID:

  NP_001136106

• Overview:

  Purified recombinant protein of Human speedy homolog A (Xenopus laevis) (SPDYA), transcript variant 3, full length, with N-terminal HIS tag, expressed in E. coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/245711

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q5MJ70

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001142634

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001136106