HLA DP (HLA-DPA1) (NM_033554) Human Recombinant Protein

• Background:

  HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq, Jul 2008]

• Synonyms:

  DP (W3) ; DP (W4) ; DPA1; HLA-DP1A; HLA-DPA; HLA-DPB1; HLADP; HLASB; PLT1

• Gene ID:

  3113

• UniProt:

  P20036, X5CKE2

• Accession Number mRNA:

  NM_033554

• Chromosomal Location:

  6p21.32

• Expression System:

  E. coli

• Tag:

  N-GST and C-His

• Related Pathways:

  Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis

• Field of Research:

  Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  50mM Tris, pH8.0, 8M Urea.

• Function:

  Transmembrane

• Molecular Weight:

  54.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  HLA-DPA1

• Species:

  Human

• Protein ID:

  NP_291032

• Overview:

  Purified recombinant protein of Human major histocompatibility complex, class II, DP alpha 1 (HLA-DPA1), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/3113

• Uniprot URL:

  https://www.uniprot.org/uniprot/P20036

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_033554

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_291032