PKC nu (PRKD3) (NM_005813) Human Recombinant Protein

• Background:

  This gene belongs to the multigene protein kinase D family of serine/threonine kinases, which bind diacylglycerol and phorbol esters. Members of this family are characterized by an N-terminal regulatory domain comprised of a tandem repeat of cysteine-rich zinc-finger motifs and a pleckstrin domain. The C-terminal region contains the catalytic domain and is distantly related to calcium-regulated kinases. Catalytic activity of this enzyme promotes its nuclear localization. This protein has been implicated in a variety of functions including negative regulation of human airway epithelial barrier formation, growth regulation of breast and prostate cancer cells, and vesicle trafficking. [provided by RefSeq, Jan 2015]

• Synonyms:

  EPK2; nPKC-NU; PKC-NU; PKD3; PRKCN

• Gene ID:

  23683

• UniProt:

  O94806

• Accession Number mRNA:

  NM_005813

• Chromosomal Location:

  2p22.2

• Expression System:

  E. coli

• Tag:

  N-His

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1% Sarkosyl.

• Function:

  Druggable Genome, Protein Kinase

• Molecular Weight:

  23.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PRKD3

• Species:

  Human

• Protein ID:

  NP_005804

• Overview:

  Purified recombinant protein of Human protein kinase D3 (PRKD3), Met300-Pro508, with N-terminal His tag, expressed in E. coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/23683

• Uniprot URL:

  https://www.uniprot.org/uniprot/O94806

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_005813

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_005804