Prothrombin (F2) (NM_000506) Human Recombinant Protein

• Background:

  This gene encodes the prothrombin protein (also known as coagulation factor II) . This protein is proteolytically cleaved in multiple steps to form the activated serine protease thrombin. The activated thrombin enzyme plays an important role in thrombosis and hemostasis by converting fibrinogen to fibrin during blood clot formation, by stimulating platelet aggregation, and by activating additional coagulation factors. Thrombin also plays a role in cell proliferation, tissue repair, and angiogenesis as well as maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in this gene lead to various forms of thrombosis and dysprothrombinemia. Rapid increases in cytokine levels following coronavirus infections can dysregulate the coagulation cascade and produce thrombosis, compromised blood supply, and organ failure. [provided by RefSeq, May 2020]

• Synonyms:

  PT; RPRGL2; THPH1

• Gene ID:

  2147

• UniProt:

  P00734

• Accession Number mRNA:

  NM_000506

• Chromosomal Location:

  11p11.2

• Expression System:

  HEK293

• Tag:

  N-His

• Related Pathways:

  Complement and coagulation cascades, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton

• Field of Research:

  Complement and coagulation cascades, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 90% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  PBS, pH7.4, 10% glycerol

• Function:

  Druggable Genome, Protease, Secreted Protein

• Molecular Weight:

  66.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  F2

• Species:

  Human

• Protein ID:

  NP_000497

• Overview:

  Purified recombinant protein of Human coagulation factor II (thrombin) (F2), esidues 44aa-end, with N-terminal His tag, expressed in human cells.

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/2147

• Uniprot URL:

  https://www.uniprot.org/uniprot/P00734

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000506

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000497