Calreticulin (CALR) Mouse Monoclonal Antibody [Clone ID: LBI13E4]

• Background:

  Calreticulin is a multifunctional protein that acts as a major Ca (2+) -binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq, Jul 2008]

• Synonyms:

  CC1qR; CRT; HEL-S-99n; RO; SSA

• Gene Name:

  Homo sapiens calreticulin (CALR), mRNA.

• Gene ID:

  811

• UniProt:

  P27797

• Conjugation:

  Unconjugated

• Related Pathways:

  Antigen processing and presentation

• Field of Research:

  Antigen processing and presentation

• Dilution:

  WB 1:500

• Form:

  Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

• Buffer:

  Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

• Function:

  Druggable Genome, Secreted Protein, Transcription Factors

• Storage Conditions:

  Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

• Symbol:

  CALR

• Overview:

  Carrier-free (BSA/glycerol-free) CALR mouse monoclonal antibody, clone LBI13E4

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/?term=811

• Uniprot URL:

  https://www.uniprot.org/uniprot/P27797