RACGAP1 Antibody (N-term) [APR30794G]

• Background:

  Tissue Location: Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase

• Synonyms:

  HGNC:9804

• Gene Name:

  HGNC:9804

• Gene ID:

  29127

• UniProt:

  Q9H0H5

• Chromosomal Location:

  Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle Cytoplasmic vesicle, secretory vesicle, acrosome. Cleavage furrow Midbody, Midbody ring. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity) . During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody ring, also called Flemming body. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase.

• Applications:

  WB, IHC-P, IF, FC

• Dilution:

  WB--1:1000 IHC-P--1:100~500 IF--1:10~50 FC--1:10~50

• Form:

  Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

• Function:

  Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.

• Molecular Weight:

  71027

• Storage Conditions:

  Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

• Overview:

  We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RACGAP1 Antibody (N-term) [APR30794G].

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/?term=29127

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9H0H5