Der p 23 Protein, Dermatophagoides pteronyssinus, Recombinant (His Tag)

• Background:

  Der p 23 is a new major allergen of D. pteronyssinus. The house dust mite (HDM) Dermatophagoides pteronyssinus is one of most important allergen sources and a major elicitor of allergic asthma. Der p 23 may be an essential component for diagnosis and specific immunotherapy of HDM allergy.

• Synonyms:

  Derp 23 Protein, Dermatophagoides pteronyssinus; Derp23 Protein, Dermatophagoides pteronyssinus

• Gene Name:

  Endochitinase-like

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant Dermatophagoides pteronyssinus DERP23 consists of 80 amino acids and predicts a molecular mass of 9.4 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  LOC113788516

• Species:

  Dermatophagoides pteronyssinus

• Protein ID:

  ACB46292.1

• Overview:

  A DNA sequence encoding the Dermatophagoides pteronyssinus DERP23 (ACB46292.1) (Met1-Thr90) was expressed with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Thr90

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/ACB46292.1