Zika virus (ZIKV) (strain Zika SPH2015) E / Envelope protein (aa 291-696, His Tag)

• Background:

  Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF., likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20 mM Tris, 300 mM NaCl, pH 8.0, 10 % glycerol. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Viral Proteins, ZikaSPH2015, ZIKV

• Molecular Weight:

  The recombinant Zika virus (strain Zika SPH2015) E consists of 417 amino acids and predicts a molecular mass of 45.6 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ZIKV-E

• Species:

  ZIKV

• Protein ID:

  ALU33341.1

• Overview:

  A DNA sequence encoding the Zika virus (strain Zika SPH2015) E (ALU33341.1, with mutation Thr 366 Ala, Gln 367 Gly, Trp 391 Arg, Leu 397 Arg) (Ile291-Thr696) was expressed with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Ile291-Thr696 (T366A, Q367G, W391R, L397R)

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/ALU33341.1