IL13RA2 Protein, Canine, Recombinant (hFc Tag)

• Background:

  Interleukin-13 receptor subunit alpha-2 (IL13RA2/IL-13RA2) is also known as cluster of differentiation 213A2 (CD213A2), IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, and IL-13RA2. The IL13RA2 is often overexpressed in brain tumors, making Il13ra2 one of the vaccine targets for immunotherapy of glioma. IL13RA2/IL-13RA2 is a cancer-associated receptor that is present in greater than 80% of High-Grade Astrocytomas (HGA) and has recently been recognized as a cytokine that predisposes breast cancer cells to metastasize. Expression of IL13Rα2 was rapidly lost from the surface of transduced cells grown in culture. The loss appeared to be related to ligands present in fetal bovine serum in the medium. None of the malignant glioma cell lines cultivated in vitro and tested to date exhibited the IL13Rα2 receptor. A recombinant virus (R5111) enters cells via its interaction with the IL13Rα2 receptor in a manner that cannot be differentiated from the interaction of wild-type virus with its receptors.

• Gene Name:

  Interleukin 13 receptor, alpha 2

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to inhibit IL13-dependent proliferation of TF-1 human erythroleukemic cells.The EDfor this effect is typically 0.01-0.04 μg/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  CD Molecule proteins, CAR T Cell Therapy Target Proteins, Receptor Proteins, Cytokine Proteins, Interleukin proteins, Biological Target proteins

• Molecular Weight:

  The recombinant canine IL13RA2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 558 amino acids and has a predicted molecular mass of 64.1 kDa. The apparent molecular mass of the protein is approximately 66-72 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IL13Ra2

• Species:

  Canine

• Protein ID:

  NP_001003075.1

• Overview:

  A DNA sequence encoding the canine IL13RA2 (NP_001003075.1) (Met1-Thr338) was expressed with the Fc region of human IgG1 at the C-terminus.

• AA Sequence:

  Met1-Thr338

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_001003075.1