PDGFA Protein, Canine, Recombinant (hFc Tag)

• Background:

  Platelet-derived growth factor alpha (PDGFA) is frequently upregulated in various cancers and thought to function as a key player in the development and progression of tumor growth by regulating aspects of cell proliferation, angiogenesis and metastasis. The human platelet-derived growth factor A chain gene (PDGFA) on chromosome 7p22 encodes an important mitogen. Within PDGFA lies a complex minisatellite structure that results in partial duplications of exon 4 and the IVS4 splice donor site.

• Gene Name:

  Platelet-derived growth factor alpha polypeptide

• Expression System:

  HEK293 Cells

• Tag:

  N-hFc

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts.The EDfor this effect is typically 5-25 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Growth Factor proteins, Cell Therapy proteins, Biological Target proteins, Cytokine Proteins

• Molecular Weight:

  The recombinant canine PDGFA consists 370 amino acids and predicts a molecular mass of 41 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PDGFA

• Species:

  Canine

• Protein ID:

  NP_001177101.1

• Overview:

  A DNA sequence encoding the canine PDGFA (NP_001177101.1) (Ser87-Arg196) was expressed with the Fc region of human IgG1 at the N-terminus.

• AA Sequence:

  Ser87-Arg196

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_001177101.1