TrkA Protein, Canine, Recombinant (hFc Tag)

• Background:

  TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by the pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in the TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation, and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to, neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF) . Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as the establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.

• Gene Name:

  Neurotrophic tyrosine kinase, receptor, type 1

• Expression System:

  HEK293 Cells

• Tag:

  N-hFc

• Field of Research:

  Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to inhibit NGF-induced proliferation of TF 1 human erythroleukemic cells.The EDfor this effect is typically 2-8 μg/mL in the presence of 10 ng/mL of recombinant human NGF.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Enzyme Proteins, Growth Factor proteins, Receptor Proteins, Cancer proteins

• Molecular Weight:

  The recombinant canine NTRK1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 386 amino acids and has a predicted molecular mass of 42.3 kDa. The apparent molecular mass of the protein is approximately 55-61 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  NTRK1

• Species:

  Canine

• Protein ID:

  XP_851619.3

• Overview:

  A DNA sequence encoding the canine NTRK1 (XP_851619.3) (Pro285-Lys410) was expressed with the Fc region of human IgG1 at the N-terminus.

• AA Sequence:

  Pro285-Lys410

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/XP_851619.3