SARS-CoV-2 (2019-nCoV) Spike S1 (Q677H) Protein (His Tag)

• Background:

  The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Immobilized human ACE2 protein (mFc tag) (Cat:PH52212M4) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1 (Q677H) Protein (His Tag) (Cat:PO54673M2), the ECof SARS-CoV-2 Spike S1 (Q677H) Protein (His Tag) (Cat:PO54673M2) is 100-600 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  2019-nCoV, HCoV

• Molecular Weight:

  The recombinant SARS-CoV-2 (2019-nCoV) Spike S1 consists of 681 amino acids and predicts a molecular mass of 76.5 kDa. As a result of glycosylation, it migrates as an approximately 112.2 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Spike

• Species:

  SARS-CoV-2

• Protein ID:

  YP_009724390.1

• Overview:

  A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike S1 (YP_009724390.1, with mutation Q677H) (Met1-Arg685) was expressed with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Arg685 (Q677H)

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/YP_009724390.1