Human respiratory syncytial virus (RSV) (B, strain 18537) Nucleoprotein Protein (His Tag)

• Background:

  Respiratory syncytial virus (RSV), also called human respiratory syncytial virus (hRSV), can cause infections of the lungs and respiratory tract. RSV also cause more severe infections such as bronchiolitis, an inflammation of the small airways in the lung, and pneumonia, an infection of the lungs. RSV is a negative-sense, single-stranded RNA virus. Its nucleocapsid (NC), which comprises the negative sense RNA viral genome coated by the viral nucleoprotein N, is a critical assembly that serves as template for both mRNA synthesis and genome replication. Nucleoprotein (RSV) play important roles during RSV replication and transcription, thereby providing further targets for antiviral research.

• Expression System:

  E. coli

• Tag:

  N-His

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 50mM PB, 500mM NaCl, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant Human respiratory syncytial virus (RSV) (B, strain 18537) Nucleoprotein consists of 398 amino acids and predicts a molecular mass of 44.38 kDa. It migrates as an approximately 41.69 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  RSV-NP

• Species:

  RSV

• Overview:

  A DNA sequence encoding the Human respiratory syncytial virus (RSV) (B, strain 18537) Nucleoprotein (P24566) (Met1-Leu391) was expressed with a polyhistidine tag at the N-terminus.

• AA Sequence:

  Met1-Leu391