CD226 Protein, Cynomolgus, Recombinant (His Tag)

• Background:

  The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD226, also known as PTA1 or DNAM-1, is a member of the immunoglobulin superfamily containing 2 Ig-like domains of the V-set. High rate of CD226 (Cluster of Differentiation 226) is found on the surface of natural killer cells, platelets, monocytes and a subset of T cells. CD226 have binding sites with CD112 and CD155 and mediate cellular adhesion to other cells containing its ligands.

• Gene Name:

  CD226 molecule

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  ITIM/ITAM Immunoreceptors and Related Molecules Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured by its binding ability in a functional ELISA. Immobilized Human CD155/PVR (hFc & AVI Tag), Biotinylated (Cat: 10109-H41H-B) at 2 μg/mL (100 μL/well) can bind Cynomolgus CD226 His (Cat:PK54014M2), the ECof Cynomolgus CD226 His (Cat:PK54014M2) is 80-480 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant cynomolgus CD226 consists of 245 amino acids and predicts a molecular mass of 28 kDa. As a result of glycosylation, it migrates as an approximately 45.7 KDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CD226

• Species:

  Cynomolgus

• Protein ID:

  A0A2K5W1Z6-1

• Overview:

  A DNA sequence encoding the cynomolgus CD226 (A0A2K5W1Z6-1) (Met1-Phe252) was expressed with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Phe252

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/A0A2K5W1Z6-1