Her2/ERBB2 Protein, Cynomolgus, Recombinant (ECD, hFc Tag)

• Background:

  Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

• Gene Name:

  Erb-b2 receptor tyrosine kinase 2

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Field of Research:

  Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  1. Immobilized Her2/ERBB2 Protein, Cynomolgus, Recombinant (ECD, hFc Tag) (Cat:PK53965M4) at 2 μg/ml (100 μl/well) can bind Anti-Erbb2 Antibody (Trastuzumab) (Cat:68046-H002), the ECis 15-70 ng/mL. 2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The EDfor this effect is 0.3-1.8 μg/mL in the presence of 0.6 μg/mL Anti-ErbB2/Her2 Monoclonal Antibody.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Kinase proteins, Growth Factor proteins, CD Molecule proteins, CAR T Cell Therapy Target Proteins, Receptor Proteins, Cancer proteins, Immunotherapy proteins, Biomarker Proteins, Enzyme Proteins, Biological Target proteins, Diagnostic proteins

• Molecular Weight:

  The recombinant cynomolgus ERBB2 consists 871 amino acids and predicts a molecular mass of 96.4 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ERBB2

• Species:

  Cynomolgus

• Overview:

  A DNA sequence encoding the cynomolgus ERBB2 (Met1-Thr652) [ Rhesus ERBB2 (XP_001090430.1, Asp121Asn, Leu122Pro) ; similar to Macaca fascicularis ERBB2 (EHH58073.1) ], was expressed with the Fc region of human IgG1 at the C-terminus.

• AA Sequence:

  Met1-Thr652