Interferon alpha 1/IFNA1 Protein, Rat, Recombinant (hFc Tag)

• Background:

  IFNA1, also known as IFN-alpha and IFNA, belongs to the alpha/beta interferon family. Interferons (IFNs) are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites, or tumor cells. They belong to the large class of glycoproteins known as cytokines. IFNs stimulate the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They allow for communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. IFNs can activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes, and they also increase the ability of uninfected host cells to resist new infection by the virus. Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon is produced by mitogen- or antigen-stimulated T lymphocytes. IFNA1 is produced by macrophages and has antiviral activities.

• Gene Name:

  Interferon, alpha 1

• UniProt:

  P05011

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV) .The EDfor this effect is 1-10 μg/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Growth Factor proteins, Immunotherapy proteins, Biomarker Proteins, Biological Target proteins, Cell Therapy proteins

• Molecular Weight:

  The recombinant rat IFNA1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 410 amino acids and predicts a molecular mass of 46.4 kDa. The apparent molecular mass of the rat IFNA1/Fc monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IFNA1

• Species:

  Rat

• Overview:

  A DNA sequence encoding the rat IFNA1 (P05011) (Met 1-Ser 192) was fused with the Fc region of human IgG1 at the C-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/P05011

• AA Sequence:

  Met1-Ser192