Products for Research Use Only
RegisterLogin

Cripto Protein, Rat, Recombinant (His Tag)

CAT: 0882-PR53671M2Size: 50 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0882-PR53671M2Size:50 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Cripto/TDGF1 is a member of the epidermal growth factor (EGF) - Cripto, Frl-1, and Cryptic (CFC) family. EGF-CFC family member proteins share a variant EGF-like motif, a conserved cysteine-rich domain, and a C-terminal hydrophobic region. Before gastrulation, Cripto is asymmetrically expressed in a proximal–distal gradient in the epiblast, and subsequently is expressed in the primitive streak and newly formed embryonic mesoderm. These proteins play key roles in intercellular signaling pathways during vertebrate embryogenesis. Mutations in Cripto/TDGF1 can cause autosomal visceral heterotaxy. Cripto/TDGF1 is involved in left-right asymmetric morphogenesis during organ development. Cripto signalling is essential for the conversion of a proximal–distal asymmetry into an orthogonal anterior–posterior axis. The mechanism of inhibitory effects of the Cripto includes both cancer cell apoptosis, activation of c-Jun-NH (2) -terminal kinase and p38 kinase signaling pathways and blocking of Akt phosphorylation. Thus, Cripto is a unique target, and Immunohistochemistry to Cripto could be of therapeutic value for human cancers.
Overview
A DNA sequence encoding the rat TDGF1 (XP_001056317.2) (Met1-Cys143) was expressed with a polyhistidine tag at the C-terminus.
Gene Name
Teratocarcinoma-derived growth factor 1
Expression System
HEK293 Cells
Tag
C-His
Field of Research
Cardiac Stem Cell Markers
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 80 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
TGF beta Family proteins, Receptor Proteins, Cytokine Proteins, Growth Factor proteins, Biological Target proteins
Molecular Weight
The recombinant rat TDGF1 comprises 138 amino acids and predicts a molecular mass of 15.5 kDa. The apparent molecular mass of the recombinant protein is approximately 22 and 19 kDa in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
TDGF1
Species
Rat
Protein ID
XP_001056317.2
AA Sequence
Met1-Cys143
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/XP_001056317.2

Related Products

CatalogName
PH51001M2Cripto Protein, Human, Recombinant (His Tag)
PH51115E1CRIPT Protein, Human, Recombinant (His Tag)
PR53901M2Reg3A Protein, Rat, Recombinant (His Tag)
PR53900M2CD36 Protein, Rat, Recombinant (His Tag)
PR53899I2IL21 Protein, Rat, Recombinant (His Tag)
PR53888E1LDHA Protein, Rat, Recombinant (His Tag)
PR53887M1CLEC5A Protein, Rat, Recombinant (His Tag)
PR53847M2LAYN Protein, Rat, Recombinant (His Tag)
PR53838M2CD4 Protein, Rat, Recombinant (His Tag)
PR53835M2EGFR Protein, Rat, Recombinant (His Tag)

Alternative Products

CatalogName
049641-01Antithrombin (AT), Recombinant, Rat, His-Tag
P7361-10μgRecombinant Rat Tropic1808, His-tag
101299MCL1, His-Tag (Rat) Recombinant
MBS567200-01RBP4 (rat), (recombinant) (His-tag)
054187-01Calcitonin (CT), Recombinant, Rat, His-Tag, SUMO-Tag
056528-01Granulin (GRN), Recombinant, Rat, His-Tag, GST-Tag
590756-01Calcitonin, Recombinant, Rat, TRX-Tag, His-Tag discontinued
054410-01Nephrin (NPHN), Recombinant, Rat, His-Tag, GST-Tag
054289-01Glucokinase (GCK), Recombinant, Rat, His-Tag, GST-Tag
050405-01Adiponectin (ADPN), Recombinant, Rat, His-Tag, SUMO-Tag

Popular Products