IL-6 Protein, Rat, Recombinant

• Background:

  Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.

• Gene Name:

  Interleukin 6

• Expression System:

  E. coli

• Tag:

  No tag

• Field of Research:

  Neuroinflammation Cancer Drug Targets

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells.The EDfor this effect is typically 1-6 ng/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 40% acetonitrile, 0.1% TFA Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Growth Factor proteins, Interleukin proteins, Biological Target proteins, Diagnostic proteins, Immunotherapy proteins, Biomarker Proteins, Cell Therapy proteins

• Molecular Weight:

  The recombinant rat IL6 consists of 188 amino acids and has a predicted molecular mass of 21.9 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 22 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IL6

• Species:

  Rat

• Protein ID:

  NP_036721.1

• Overview:

  A DNA sequence encoding the rat IL6 (NP_036721.1) (Phe25-Thr211) was expressed and purified with an initial Met.

• AA Sequence:

  Phe25-Thr211

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_036721.1