SPARCL1 Protein, Mouse, Recombinant (His Tag)

• Background:

  SPARC-like protein 1 (SPARCL1; also known as SC1, high endothelial venule protein, or hevin) is an extracellular matrix-associated, secreted glycoprotein belonging to the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins. It contains three conserved structural domains that are implicated in the regulation of cell adhesion, migration, and proliferation. SPARCL1 is expressed during embryogenesis and tissue remodeling and is especially prominent in brain and vasculature. Its down-regulation in a number of cancers and the possibility of its functional compensation by SPARC has led to recent interest in hevin as a tumor suppressor and regulator of angiogenesis. SPARCL1 has antiadhesive properties, and loss of SPARCL1 expression is associated with increased proliferative activity and cell cycle progression. It is suggested that it may influence multiple cellular processes during distinct stages of brain development and function. Besides, SPARCL1 can influence the function of astroglial cells in the developing and mature central nervous system (CNS) .

• Synonyms:

  Ecm2 Protein, Mouse; hevin Protein, Mouse; mast9 Protein, Mouse; Sc1 Protein, Mouse

• Gene Name:

  SPARC like 1

• Expression System:

  HEK293 Cells

• Tag:

  N-His

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The EDfor this effect is typically 1-4 μg/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant mouse Sparcl1 consists 302 amino acids and predicts a molecular mass of 34.8 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SPARCL1

• Species:

  Mouse

• Protein ID:

  NP_034227.3

• Overview:

  A DNA sequence encoding the mouse Sparcl1 (NP_034227.3) (Tyr368-Phe650) was expressed with a polyhistidine tag at the N-terminus.

• AA Sequence:

  Tyr368-Phe650

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_034227.3