EphB1 Protein, Mouse, Recombinant (His Tag)

• Background:

  Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs) . Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

• Synonyms:

  9330129L11 Protein, Mouse; AW488255 Protein, Mouse; C130099E04Rik Protein, Mouse; Cek6 Protein, Mouse; Elk Protein, Mouse; Elkh Protein, Mouse; ENSMUSG00000074119 Protein, Mouse; Hek6 Protein, Mouse; Net Protein, Mouse

• Gene Name:

  EPH receptor B1

• UniProt:

  Q8CBF3-1

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 98 % as determined by SDS-PAGE.

• Activity:

  Immobilized mouse EPHB1-His at 10 μg/ml (100 μl/well) can bind mouse EFNB1-Fc (Cat:PM52747M4), The ECof mouse EFNB1-Fc (Cat:PM52747M4) is 0.04-0.08 μg/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Kinase proteins, Growth Factor proteins, Receptor Proteins, Enzyme Proteins

• Molecular Weight:

  The secreted recombinant mouse EPHB1 consists of 533 amino acids and has a predicted molecular mass of 59.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm EPHB1 is approximately 64 kDa due to glycosylation.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  EphB1

• Species:

  Mouse

• Overview:

  A DNA sequence encoding the mouse EPHB1 (Q8CBF3-1) extracellular domain (Met 1-Leu 539) was expressed, with a polyhistidine tag at the C-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8CBF3-1

• AA Sequence:

  Met1-Leu539