P-Selectin Protein, Mouse, Recombinant (hFc Tag)

• Background:

  P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes.

• Synonyms:

  CD62P Protein, Mouse; GMP-140 Protein, Mouse; Grmp Protein, Mouse; LECAM3 Protein, Mouse; PADGEM Protein, Mouse

• Gene Name:

  Selectin P (granule membrane protein 140kDa, antigen CD62)

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse SELP/Fc Chimera coated plates (10 μg/mL, 100 μL/well), > 60% cells will adhere after 1 hour at 37°C.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Diagnostic proteins, CD Molecule proteins

• Molecular Weight:

  The recombinant mouse SELP comprises 909 amino acids and has a predicted molecular mass of 99.6 kDa. The apparent molecular mass of the protein is approximately 134.5 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SELP

• Species:

  Mouse

• Protein ID:

  NP_035477.1

• Overview:

  A DNA sequence encoding the mouse SELP (NP_035477.1) (Met1-Ala709) was expressed with the Fc region of human IgG1 at the C-terminus.

• AA Sequence:

  Met1-Ala709

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_035477.1