Thimet Oligopeptidase/THOP1 Protein, Mouse, Recombinant (His Tag)

• Background:

  THOP1, also known as Thimet oligopeptidase 1, Thimet oligopeptidase, EC 3.4.24.15, or EP24.15, is a zinc (II) endopeptidase implicated in the processing of numerous physiological peptides. As an intracellular enzyme, highly expressed in the brain, kidneys and neuroendocrine tissue, THOP1 has been proposed to metabolize peptides within cells, thereby affecting antigen presentation and G protein-coupled receptor signal transduction. Its substrates is gonadotrophin-releasing hormone (GnRH), an important hypothalamic hormone that regulates the synthesis and release of oestradiol and facilitates female sexual behaviour. THOP1 against toxic effects of Abeta in the early stages of Alzheimer disease (AD) pathology, and suggest that the observed increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load.

• Synonyms:

  AI131655 Protein, Mouse; AI327041 Protein, Mouse; EP24.15 Protein, Mouse

• Gene Name:

  Thimet oligopeptidase 1

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  N-His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl) acetyl-Pro-Leu-Gly-Pro-D-Lys (2,4-dinitrophenyl) -OH or Mca-PLGPK (Dnp) -OH.The specific activity is > 100 pmoles/min/μg.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins, Protease proteins

• Molecular Weight:

  The recombinant mouse THOP1 consists of 705 amino acids and has a calculated molecular mass of 80.1 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  THOP1

• Species:

  Mouse

• Protein ID:

  NP_073144.3

• Overview:

  A DNA sequence encoding the mouse THOP1 (NP_073144.3) (Lys 2-Cys 687) was fused with a polyhistidine tag at the N-terminus.

• AA Sequence:

  Lys2-Cys687

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_073144.3