Netrin G1/NTNG1 Protein, Human, Recombinant (His Tag)

• Background:

  NTNG1 (Netrin G1) is a Protein Coding gene. This gene encodes a preproprotein that is processed into a secreted protein containing eukaryotic growth factor (EGF) -like domains. NTNG1 is broadly expressed in the brain, kidney, and other tissues. Netrins including NTNG1 are known to be axon guidance factors in the developing brain. It is involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular, and synaptic levels. The NTNG1 protein has an important role in the developing central nervous system, particularly in axonal guidance, signaling, and NMDA receptor function, and was a good candidate gene for Rett syndrome (RTT) . They could be very important contributors to the genetic risk for psychosis.

• Synonyms:

  Lmnt1 Protein, Human

• Gene Name:

  Netrin G1

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant human NTNG1 consists 401 amino acids and predicts a molecular mass of 45.8 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  NTNG1

• Species:

  Human

• Protein ID:

  NP_055732.2

• Overview:

  A DNA sequence encoding the human NTNG1 (NP_055732.2) (Met1-Gly408) was expressed with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Gly408

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_055732.2