GDF15 Protein, Human, Recombinant (Mature Form, His Tag)

• Background:

  Growth-differentiation factor 15 (GDF15), also known as MIC-1, is a secreted member of the transforming growth factor (TGF) -β superfamily, as a novel antihypertrophic regulatory factor in the heart. GDF-15 / GDF15 is not expressed in the normal adult heart but is induced in response to conditions that promote hypertrophy and dilated cardiomyopathy and it is expressed highly in liver. GDF-15 / GDF15 has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF-15 / GDF15 is synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein. GDF-15 / GDF15 overexpression arising from an expanded erythroid compartment contributes to iron overload in thalassemia syndromes by inhibiting hepcidin expression.

• Synonyms:

  GDF-15 Protein, Human; MIC-1 Protein, Human; MIC1 Protein, Human; NAG-1 Protein, Human; PDF Protein, Human; PLAB Protein, Human; PTGFB Protein, Human

• Gene Name:

  Growth differentiation factor 15

• Expression System:

  Yeast

• Tag:

  N-His

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 50mM HAc, pH 2.9. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  TGF beta Family proteins, Cytokine Proteins, Growth Factor proteins, Biological Target proteins

• Molecular Weight:

  The recombinant mature form of human GDF15 consists of 122 amino acids and predicts a molecular mass of 13.7 kDa. As a result of glycosylation, it migrates as an approximately 18.2 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  GDF15

• Species:

  Human

• Protein ID:

  NP_004855.2

• Overview:

  A DNA sequence encoding the mature form of human GDF15 (NP_004855.2) (Ala197-Ile308) was expressed with a polyhistidine tag at the N-terminus.

• AA Sequence:

  Ala197-Ile308

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_004855.2