COL6A3 Protein, Human, Recombinant (His Tag)

• Gene Name:

  Collagen, type VI, alpha 3

• UniProt:

  P12111-1

• Expression System:

  HEK293 Cells

• Tag:

  N-His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant human COL6A3 comprises 101 amino acids and has a predicted molecular mass of 11.4 kDa. The apparent molecular mass of the protein is approximately 13.3 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  COL6A3

• Species:

  Human

• Overview:

  A DNA sequence encoding the BPTI/Kunitz inhibitor domain of human COL6A3 (P12111-1) (3101T-3177T) was expressed with an N-terminal polyhistidine tag.

• Uniprot URL:

  https://www.uniprot.org/uniprot/P12111-1

• AA Sequence:

  3101T-3177T