MSRB3 Protein, Human, Recombinant (His Tag)

• Background:

  MSRB3 (Methionine Sulfoxide Reductase B3) is a Protein Coding gene. The protein encoded by this gene catalyzes the reduction of methionine sulfoxide to methionine. This enzyme acts as a monomer and requires zinc as a cofactor. This cysteine residue of MSRB3 is conserved in orthologs from yeast to humans and is involved in binding structural zinc. The affected individuals of six unrelated families were homozygous for the same transversion (c.265T>G) in MSRB3. In the inner ear, it is found in the sensory epithelium of the organ of Corti and vestibular end organs as well as in cells of the spiral ganglion. MSRB3-catalyzed reduction of methionine sulfoxides to methionine is essential for hearing. Diseases associated with MSRB3 include Deafness, Autosomal Recessive 74, and Autosomal Recessive Non-Syndromic Sensorineural Deafness Type Dfnb.

• Synonyms:

  DFNB74 Protein, Human

• Gene Name:

  Methionine sulfoxide reductase B3

• UniProt:

  Q8IXL7-2

• Expression System:

  E. coli

• Tag:

  N-His

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant human MSRB3 consists of 178 amino acids and predicts a molecular mass of 19.6 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  MSRB3

• Species:

  Human

• Overview:

  A DNA sequence encoding the human MSRB3 (Q8IXL7-2) (Gly26-Leu185) was expressed with a polyhistide tag at the N-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8IXL7-2

• AA Sequence:

  Gly26-Leu185