Bcl-2 Protein, Human, Recombinant (His Tag)

• Background:

  BCL2 (B-cell leukemia/lymphoma 2, N-Histidine-tagged), also known as Bcl-2, belongs to the Bcl-2 family. Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes. It is expressed in a variety of tissues. BCL2 blocks the apoptotic death of some cells such as lymphocytes. It also regulates cell death by controlling the mitochondrial membrane permeability and inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

• Synonyms:

  Bcl-2 Protein, Human; PPP1R50 Protein, Human

• Gene Name:

  B-cell CLL/lymphoma 2

• UniProt:

  P10415-1

• Expression System:

  E. coli

• Tag:

  C-His

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Measured by its binding ability in a functional ELISA.Immobilized human BCL2-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse BCL2L1-His (Cat:PM52898E2), The ECof biotinylated mouse BCL2L1-His (Cat:PM52898E2) is 0.07-0.15 μg/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 50mM Tris, 20% glycerol, 100mM Arg, pH 8.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Biomarker Proteins, Diagnostic proteins, Cancer proteins

• Molecular Weight:

  The recombinant human BCL2 consisting of 221 amino acids and has a calculated molecular mass of 24.7 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  BCL2

• Species:

  Human

• Overview:

  A DNA sequence encoding the human BCL2 isoform 1 (P10415-1) (Met 1-Asp 211) was expressed, with a polyhistide tag at the C-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/P10415-1

• AA Sequence:

  Met1-Asp211