Rack1 Protein, Human, Recombinant (His & MBP Tag)

• Background:

  The scaffolding protein, Rack1, is a seven-WD-domain-containing protein that has been implicated in binding to integrin beta subunit cytoplasmic domains and to members of two kinase families (src and protein kinase C, PKC) that mediate integrin bidirectional signaling.Rack1 may link protein kinase C directly to integrins and participate in the regulation of integrin functions.Rack1 regulates the localization of an essential PCP protein and acts as a molecular switch to promote PCP signaling.

• Synonyms:

  Gnb2-rs1 Protein, Human; H12.3 Protein, Human; HLC-7 Protein, Human; PIG21 Protein, Human; RACK1 Protein, Human

• UniProt:

  P63244

• Expression System:

  E. coli

• Tag:

  N-His & MBP

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 83 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant human GNB2L1/MBP fusion protein consists of 714 amino acids and has a calculated molecular mass of 78.7 KDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Rack1

• Species:

  Human

• Overview:

  A DNA sequence encoding the human GNB2L1 (P63244) (Met 1-Arg 317) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/P63244

• AA Sequence:

  Met1-Arg317