IL22 Protein, Human, Recombinant

IL22 is a member of a group of cytokines called the IL-1 family of IL-1 superfamily (including IL-19, IL-2, IL-24, and IL-26), a class of potent mediators of cellular inflammatory responses. It shares the use of IL-1R2 in cell signaling with other members of this family, IL-1, IL-26, IL-28A/B, and IL-29. IL22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL22 along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems. IL22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-1R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (SIL-22R1) . IL22 and IL-1 receptor chains play a role in cellular targeting and signal transduction to selectively initiate and regulate immune responses. IL22 can contribute to immune disease through the stimulation of inflammatory responses, S1s, and defensins. IL22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-1. In some contexts, the pro-inflammatory versus tissue-protective functions of IL22 are regulated by the often co-expressed cytokine IL-17A.

A DNA sequence encoding human IL22 (NP_065386.1) (Ala34-Ile179) was expressed with a N-terminal Met.

IL-22 Protein, Human; IL-D110 Protein, Human; IL-TIF Protein, Human; ILTIF Protein, Human; TIFa Protein, Human; TIFIL-23 Protein, Human; zcyto18 Protein, Human

Interleukin 22

E. coli

No tag

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> 95 % as determined by SDS-PAGE.

Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The EDfor this effect is typically 0.1-0.5 ng/mL.

Lyophilized

Lyophilized from sterile PBS, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Cytokine Proteins, Growth Factor proteins, Interleukin proteins, Biological Target proteins

The recombinant human IL22 consists of 147 amino acids and predicts a molecular mass of 16.9 KDa. It migrates as an approximately 15 KDa band in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

IL22

Human

NP_065386.1

Ala34-Ile179