VEGFR3/FLT4 Protein, Human, Recombinant (His Tag)

• Background:

  Vascular endothelial growth factor receptor 3 (VEGFR3), also known as FLT-4, together with the other two members VEGFR1 (FLT-1) and VEGFR2 (KDR/Flk-1) are receptors for vascular endothelial growth factors (VEGF) and belong to the class III subfamily of receptor tyrosine kinases (RTKs) . The VEGFR3 protein is expressed mainly on lymphatic vessels but it is also up-regulated in tumor angiogenesis. Mutations in VEGFR3 have been identified in patients with primary lymphoedema. The VEGF-C/VEGF-D/VEGFR3 signaling pathway may provide a target for antilymphangiogenic therapy in prostate cancer, breast cancer, gastric cancer, lung cancer, non-small cell lung cancer (NSCLC), and so on.

• Synonyms:

  FLT-4 Protein, Human; FLT-41 Protein, Human; FLT41 Protein, Human; LMPH1A Protein, Human; PCL Protein, Human; VEGF Receptor 3 Protein, Human; VEGFR-3 Protein, Human; VEGFR3 Protein, Human

• Gene Name:

  Fms related tyrosine kinase 4

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 97 % as determined by SDS-PAGE.

• Activity:

  1. Measured by its binding ability in a functional ELISA. 2. Immobilized human VEGF-C (Cat: PH51671M2) at 10 μg/mL (100 μl/well) can bind human VEGFR3-his. The ECof human VEGFR3-his is 0.011 μg/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Kinase proteins, Growth Factor proteins, Receptor Proteins, Cancer proteins, Enzyme Proteins

• Molecular Weight:

  The recombinant human VEGF R3 consists of 763 amino acids and predicts a molecular mass of 86 kDa. As a result of glycosylation, rhVEGFR3 migrates as an approximately 130 kDa in non-reduced SDS-PAGE.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  FLT4

• Species:

  Human

• Protein ID:

  NP_002011.2

• Overview:

  A DNA sequence encoding the extracellular domain (Met 1-Ile 776) of human VEGFR3 (NP_002011.2) was expressed with a C-terminal polyhistidine tag.

• AA Sequence:

  Met1-Ile776

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_002011.2