LAMP3/CD208 Protein, Human, Recombinant (His Tag)

• Background:

  Dendritic cell-lysosomal associated membrane protein (DC-LAMP) /CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as a marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in the activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.

• Synonyms:

  CD208 Protein, Human; DC LAMP Protein, Human; DC-LAMP Protein, Human; DCLAMP Protein, Human; LAMP Protein, Human; LAMP-3 Protein, Human; TSC403 Protein, Human

• Gene Name:

  Lysosomal associated membrane protein 3

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Biomarker Proteins, Enzyme Proteins, Diagnostic proteins, CD Molecule proteins

• Molecular Weight:

  The secreted recombinant human LAMP3 consists of 365 amino acids with the predicted molecular mass of 39 kDa. As a result of glycosylation, rh LAMP3 migrates as an approximately 70-90 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  LAMP3

• Species:

  Human

• Protein ID:

  NP_055213.2

• Overview:

  A DNA sequence encoding the mature form of human LAMP3 (NP_055213.2) (Lys 28-Thr 381) with a carboxy-terminal polyhistidine tag was expressed.

• AA Sequence:

  Lys28-Thr381

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_055213.2