TSLP Protein, Human, Recombinant (hFc Tag)

• Background:

  Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7) -like cytokine originally characterized by its ability to promote the activation of B cells and dendritic cells (DCs) . Thymic stromal lymphopoietin (TSLP) is a cytokine expressed by epithelial cells, including keratinocytes, and is important in allergic inflammation. Subsequent studies have shown that TSLP promotes T helper type 2 (TH2) cell responses associated with immunity to some helminth parasites and the pathogenesis of many inflammatory diseases, including atopic dermatitis and asthma. TSLP can promote TH2 cytokine-associated inflammation by directly promoting the effector functions of CD4TH2 cells, basophils and other granulocyte populations while simultaneously limiting the expression of DC-derived proinflammatory cytokines and promoting regulatory T cell responses in peripheral tissues.

• Gene Name:

  Thymic stromal lymphopoietin

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Immobilized Recombinant Human CRLF2 Protein (His Tag) (Cat:PH50731M2) at 2 μg/mL (100 μL/well) can bind Recombinant Human TSLP Protein (R127A, R130S, Fc Tag) (Cat:PH51745M4), the ECis 1.5-4.0 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant Human TSLP consists of 369 amino acids and predicts a molecular mass of 41.50 kDa. It migrates as an approximately 46.22 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  TSLP

• Species:

  Human

• Overview:

  A DNA sequence encoding the Human TSLP (NP_149024.1) (Met1-Gln159) was expressed with the fused Fc region of human IgG1 at the C-terminus.

• AA Sequence:

  Met1-Gln159