HAI-2/SPINT2 Protein, Human, Recombinant (ECD, hFc Tag)

• Background:

  Choanal (CA) and gastrointestinal atresias (GA) are an important feature of syndromic congenital sodium diarrhea (sCSD), a disorder recently associated with mutations in the gene for serine protease inhibitor type 2 (SPINT2) . The SPINT2 gene is epigenetically silenced or downregulated in human cancers, altering the balance of HGF activation/inhibition ratio, which contributes to cancer development and progression. SPINT2 is a tumor suppressor gene that inhibits proteases implicated in cancer progression, like HGFA, hepsin and matriptase. Loss of SPINT2 expression in tumors has been associated with gene promoter hypermethylation. SPINT2 (serine peptidase inhibitor Kunitz type 2), a proteolytic inhibitor of hepatocyte growth factor activator (HGFA), has a significant role in the suppression of the HGF-MET pathway and malignant melanoma progression.

• Synonyms:

  DIAR3 Protein, Human; HAI-2 Protein, Human; HAI2 Protein, Human; Kop Protein, Human; PB Protein, Human

• Gene Name:

  Serine peptidase inhibitor, Kunitz type, 2

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Field of Research:

  Serine Proteases and Regulators

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys (DNP) -NH2.The IC50 value is < 1 nM.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant human SPINT2 consists 408 amino acids and predicts a molecular mass of 45.9 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SPINT2

• Species:

  Human

• Protein ID:

  NP_066925.1

• Overview:

  A DNA sequence encoding the human SPINT2 (NP_066925.1) (Met1-Lys197) was expressed with the Fc region of human IgG1 at the C-terminus.

• AA Sequence:

  Met1-Lys197

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_066925.1