14-3-3 epsilon Protein, Human, Recombinant (GST Tag)

• Background:

  YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner.

• Synonyms:

  14-3-3E Protein, Human; HEL2 Protein, Human; KCIP-1 Protein, Human; MDCR Protein, Human; MDS Protein, Human

• Gene Name:

  Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon

• Expression System:

  E. coli

• Tag:

  N-GST

• Field of Research:

  Phospho-Serine/phospho-Threonine Binding Proteins Adaptor Proteins Apoptosis Adaptor Proteins

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 92 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.25mM DTT, 25% glycerol, 0.5mM GSH, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant human YWHAE/GST chimera consists of 487 amino acids and has a predicted molecular mass of 56 kDa. It migrates as an approxiamtely 50 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  YWHAE

• Species:

  Human

• Protein ID:

  NP_006752.1

• Overview:

  A DNA sequence encoding the human YWHAE (NP_006752.1) (Met 1-Gln 255) was fused with the GST tag at the N-terminus.

• AA Sequence:

  Met1-Gln255

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_006752.1