GM-CSF Receptor alpha Protein, Human, Recombinant (His Tag)

• Background:

  CD116/GM-CSFR has been preferentially associated with M4, M5 subtype of AML but is not specific. The cluster of differentiation (cluster of designation) (often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules present on white blood cells initially but found in almost any kind of cell of the body, providing targets for immunophenotyping of cells. Physiologically, CD molecules can act in numerous ways, often acting as receptors or ligands (the molecule that activates a receptor) important to the cell. A signal cascade is usually initiated, altering the behavior of the cell (see cell signaling) . Some CD proteins do not play a role in cell signaling, but have other functions, such as cell adhesion. CD116/GM-CSFR is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. CD116/GM-CSFR is found in the pseudoautosomal region (PAR) of the X and Y chromosomes.

• Synonyms:

  CD116 Protein, Human; CDw116 Protein, Human; CSF2R Protein, Human; CSF2RAX Protein, Human; CSF2RAY Protein, Human; CSF2RX Protein, Human; CSF2RY Protein, Human; GM-CSF-R-alpha Protein, Human; GMCSFR Protein, Human; GMR Protein, Human; SMDP4 Protein, Human

• Gene Name:

  Colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)

• UniProt:

  P15509-1

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The EDfor this effect is 3-10 μg/mL in the presence of 0.4ng/mL GMCSF.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  CD Molecule proteins, Receptor Proteins, Cytokine Proteins, Interleukin proteins

• Molecular Weight:

  The recombinant human GM-CSFRα consists of 309 amino acids and has a predicted molecular mass of 36 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh GM-CSFRα is approximately 60-65 kDa due to glycosylation.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CSF2RA

• Species:

  Human

• Overview:

  A DNA sequence encoding the extracellular domain (Met 1-Gly 320) of human GM-CSFRα (P15509-1) was expressed with the C-terminal fused polyhistidine tag.

• Uniprot URL:

  https://www.uniprot.org/uniprot/P15509-1

• AA Sequence:

  Met1-Gly320