CD96 Protein, Human, Recombinant (His Tag), Biotinylated

• Background:

  The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD155 ligand CD96 is a member of the Ig superfamily. It's an immunoglobulin-like protein tentatively allocated to the repertoire of human NK receptors. NK cells recognize poliovirus receptor (PVR), a nectins and nectin-like protein family member serve to mediate cell-cell adhesion, cell migration, with the presence of an additional receptor, CD96. CD96 promotes NK cell adhesion to target cells expressing PVR, stimulates cytotoxicity of activated NK cells, and mediates acquisition of PVR from target cells. The effect the cells with mutated CD96 protein lost adhesion and growth activities indicates that CD96 mutations may cause a form of the C syndrome by interfering with cell adhesion and growth.

• Synonyms:

  TACTILE Protein, Human

• Gene Name:

  CD96 molecule

• UniProt:

  P40200-2

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  Immobilized human CD155/PVR (Fc Tag) (PH50960M4) at 2 μg/mL (100 μL/well) can bind CD96 (His Tag), Biotinylated (PH51060M2), the ECof CD96 (His Tag), Biotinylated (PH51060M2) is 150-800 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Biomarker Proteins, Immune Checkpoint Proteins, Biological Target proteins, CD Molecule proteins, Diagnostic proteins, Biotinylated proteins, Cancer proteins

• Molecular Weight:

  The recombinant human CD96 consists of 493 amino acids and predictes a molecular mass of 55 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD96 is approximately 120-130 kDa due to high glycosylation.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CD96

• Species:

  Human

• Overview:

  A DNA sequence encoding the human CD96 isoform 2 (P40200-2) extracellular domain (Met1-Met503) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylatedin vitro.

• Uniprot URL:

  https://www.uniprot.org/uniprot/P40200-2

• AA Sequence:

  Met1-Met503