Sphingosine Kinase 1 Protein, Human, Recombinant

• Background:

  SPHK1, also known as sphingosine Kinase 1, catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P) . S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.

• Synonyms:

  SPHK Protein, Human; SPHK1 Protein, Human; SPK Protein, Human

• Gene Name:

  Sphingosine kinase 1

• UniProt:

  Q9NYA1-1

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  No tag

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  The specific activity was determined to be > 500nmol/min/mg using Sphingosine Kinase Substrate as substrate.

• Form:

  Liquid

• Buffer:

  Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins

• Molecular Weight:

  The recombinant human SPHK1 consists of 386 amino acids and has a calculated molecular mass of 42.7 kDa. The recombinant protein migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SPHK1

• Species:

  Human

• Overview:

  A DNA sequence encoding the human SPHK1 (Q9NYA1-1) (Met1-Leu384) was fused with two additional amino acids (Gly & Pro) at the N-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9NYA1-1

• AA Sequence:

  Met1-Leu384