Products for Research Use Only
RegisterLogin

GDNF Protein, Human, Recombinant

CAT: 0882-PH51024M0-01Size: 10 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0882-PH51024M0-01Size:10 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Glial cell line-derived neurotrophic factor (GDNF) is an important member of the GDNF family of ligands (GFL) . The GDNF family of ligands is comprised by four neurotrophic factors: glial cell line-derived neurotrophic factor (GDNF), neurturin (NRTN), artemin (ARTN), and persephin (PSPN) . It has been found that GFLs play a role in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration. As the founding member, GDNF plays a key role in the promotion of the survival of dopaminergic neurons. GDNF is a highly conserved neurotrophic factor. The recombinant form of this protein also promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. GDNF also regulates kidney development and spermatogenesis, and it affects alcohol consumption. It has been shown that GDNF results in two Parkinson's disease clinical trial and in a number of animal trials. It has been taken as a potent survival factor for central motoneurons.
Overview
A DNA sequence encoding the mature form of human GDNF (P39905-2) (Arg 83-Ile 185) was expressed and purified, with additional two aa (Gly& Pro) at the N-terminus.
Synonyms
ATF1 Protein, Human; ATF2 Protein, Human; HFB1-GDNF Protein, Human; HSCR3 Protein, Human
Gene Name
Glial cell derived neurotrophic factor
UniProt
P39905-2
Expression System
HEK293 Cells
Tag
No tag
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 97 % as determined by SDS-PAGE.
Activity
1. Measured in a cell proliferation assay using SH SY5Y human neuroblastoma cells. The EDfor this effect is typically 3-20 ng/mL in the presence of Recombinant Human GFR alpha 1/GDNF R alpha 1 His Chimera. 2. Immobilized Recombinant Human GDNF Protein (Cat:PH51024M0) at 2 μg/mL (100 μL/well) can bind Recombinant Human GFRA1 / GFRα1 / GDNFRA Protein (His Tag) (Cat:PH51063M2), the ECis 15-48 ng/mL.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Cytokine Proteins, Growth Factor proteins, TGF beta Family proteins, Biological Target proteins
Molecular Weight
The recombinant human GDNF consists of 105 amino acids and predicts a molecular mass of 15.1 KDa. It migrates as approximately 18 KDa band in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
GDNF
Species
Human
Uniprot URL
https://www.uniprot.org/uniprot/P39905-2
AA Sequence
Arg83-Ile185

Related Products

CatalogName
PH0113M2Recombinant Human GDNF Protein
PH31915E1GDNF (NM_199234) Human Recombinant Protein
PH30747E1GDNF (NM_199231) Human Recombinant Protein
PR53644I1GDNF Protein, Rat, Recombinant (His Tag)
PO54441M3GDNF Protein, Canine, Recombinant (hFc Tag)
PH33397M5GDNF Receptor alpha 1 (GFRA1) (NM_001145453) Human Recombinant Protein
APR12013GPolyclonal GDNF Antibody
APR12014GPolyclonal GDNF Antibody
APR12015GPolyclonal GDNF Antibody
LH02954VGDNF (NM_000514) Human Over-expression Lysate

Alternative Products

CatalogName
NBP2-34888-10ugRecombinant Human GDNF Protein
OPCA03860-1MGGDNF Recombinant Protein (Human)
PROTP39905-2-250ugHuman recombinant GDNF protein
CST 16187SHuman GDNF Recombinant Protein
E45H51024M0-10GDNF Protein, Human, Recombinant

Popular Products