CD200 Protein, Human, Recombinant (His Tag)

• Background:

  CD200 (OX-2) is a cell surface glycoprotein that imparts immune privileges by suppressing alloimmune and autoimmune responses through its receptor, CD200R, expressed primarily on myeloid cells. Signals delivered through the CD200:CD200R axis have been shown to play an important role in the regulation of anti-tumor immunity, and overexpression of CD200 has been reported in a number of malignancies, including CLL, as well as on cancer stem cells. The role of CD200-CD200R signaling in immune regulation of the central nervous system has become a popular field of research in recent years. Many studies have shown that there is a close correlation between CD200-CD200R, microglia activation, and Parkinson's disease (PD) . The ability of CD200 to suppress myeloid cell activation is critical for maintaining normal tissue homeostasis but may also enhance the survival of migratory neoplastic cells. CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 has been characterized as an important immunoregulatory molecule, increased expression of which can lead to decreased transplant rejection, autoimmunity, and allergic disease. Elevated CD200 expression has been reported to be associated with poor prognosis in some human malignancies. Besides, CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.

• Synonyms:

  MOX1 Protein, Human; MOX2 Protein, Human; MRC Protein, Human; OX-2 Protein, Human; OX2 Protein, Human

• Gene Name:

  Cd200 molecule

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Neuroinflammation Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 98 % as determined by SDS-PAGE.

• Activity:

  Immobilized CD200 Protein, Human, Recombinant (His Tag) (Cat:PH50974M2) at 2 μg/mL (100 μL/well) can bind CD200R Protein, Human, Recombinant (His & hFc Tag) (Cat:PH50521M4), the ECis 5-28ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  CD Molecule proteins, Immunotherapy proteins, Cancer proteins, Immune Checkpoint Proteins

• Molecular Weight:

  The mature recombinant human CD200 consists of 213 amino acids and predicts a molecular mass of 24 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rhC200 is approximately 40-45 kDa due to glycosylation.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CD200

• Species:

  Human

• Protein ID:

  NP_005935.4

• Overview:

  A DNA sequence encoding the human CD200 (NP_005935.4) extracellular domain (Met 1-Gly 232) was fused with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Gly232

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_005935.4