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CD81 Protein, Human, Recombinant

CAT: 0882-PH50937M0Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0882-PH50937M0Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
CD81, also known as TAPA-1, belongs to the transmembrane 4 superfamily, also known as the tetraspanin family. Members of this family mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.CD81 is a widely expressed cell-surface protein involved in an astonishing variety of biologic responses. It is related to adhesion, morphology, activation, proliferation, and differentiation of B, T, and other cells. On B cells CD81 is part of a complex with CD21, CD19, and Leu13. This complex reduces the threshold for B cell activation via the B cell receptor by bridging Ag specific recognition and CD21-mediated complement recognition.
Overview
A DNA sequence encoding the human CD81 (NP_004347.1) (Phe113-Lys201) was expressed with two additional amino acids (Gly&Pro) at the N-terminus.
Synonyms
CVID6 Protein, Human; S5.7 Protein, Human; TAPA1 Protein, Human; TSPAN28 Protein, Human
Gene Name
CD81 molecule
Expression System
HEK293 Cells
Tag
No tag
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
CD Molecule proteins
Molecular Weight
The recombinant human CD81 comprises 91 amino acids and has a predicted molecular mass of 9.9 kDa. The apparent molecular mass of the monomer is approximately 10 kDa in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
CD81
Species
Human
Protein ID
NP_004347.1
AA Sequence
Phe113-Lys201
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_004347.1

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