Endothelin B Receptor Protein, Human, Recombinant (mFc Tag)

• Background:

  The hypermethylation of EDNRB gene was remarkably related to infiltration and metastasis of gastric cancer and may attribute to the tumor progression. EDNRB is a new candidate tumor suppressor gene which is often down-regulated or even silenced by promoter hypermethylation in various human cancers. Low EDNRB expression played a role in the progression of ACC tumors. The autosomal recessive mutation in EDNRB may underlie a part of WS1 with the current diagnostic criteria, and supported that Hirschsprung's disease is a multifactorial genetic disease which requires additional factors.

• Synonyms:

  ABCDS Protein, Human; ET-B Protein, Human; ET-BR Protein, Human; ETB Protein, Human; ETBR Protein, Human; ETRB Protein, Human; HSCR Protein, Human; HSCR2 Protein, Human; WS4A Protein, Human

• Gene Name:

  Endothelin receptor type B

• Expression System:

  HEK293 Cells

• Tag:

  C-mFc

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant human EDNRB/mFc comprises 309 amino acids and has a predicted molecular mass of 34.4 kDa. The apparent molecular mass of the protein is approximately 47.5 and 43.7 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  EDNRB

• Species:

  Human

• Protein ID:

  NP_000106.1

• Overview:

  A DNA sequence encoding the human EDNRB (NP_000106.1) (Met1-Lys101) was expressed with the Fc region of mouse IgG1 at the C-terminus.

• AA Sequence:

  Met1-Lys101

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_000106.1