CD38 Protein, Human, Recombinant (ECD, hFc Tag)

• Background:

  The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 38 (CD38), also known as ADP-ribosyl cyclase, is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4, CD8, B and natural killer cells. It shares several characteristics with ADP-ribosyl cyclase 2 CD157. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NADto ADP-ribose. It also functions in cell adhesion, signal transduction and calcium signaling. CD38 has been used as a prognostic marker in leukemia. It can also be used to identify plasma cells.

• Synonyms:

  ADPRC 1 Protein, Human; ADPRC1 Protein, Human; T10 Protein, Human

• Gene Name:

  CD38 molecule

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cancer proteins, Biomarker Proteins, Biological Target proteins, Diagnostic proteins, CD Molecule proteins, CAR T Cell Therapy Target Proteins, Receptor Proteins

• Molecular Weight:

  The recombinant human CD38 consists of 496 amino acids and predicts a molecular mass of 56.6 kDa. As a result of glycosylation, human CD38 migrates as an approximately 64.7 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CD38

• Species:

  Human

• Protein ID:

  NP_001766.2

• Overview:

  A DNA sequence encoding the human CD38 (NP_001766.2) (Val43-Ile300) was expressed with the Fc region of human IgG1 at the C-terminus.

• AA Sequence:

  Val43-Ile300

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_001766.2