CK2 alpha/CSNK2A1 Protein, Human, Recombinant (GST Tag)

• Background:

  Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser / Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine / threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine / threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha'-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB) . CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via the phosphorylation of Akt.

• Synonyms:

  CK2A1 Protein, Human; CKII Protein, Human; CSNK2A3 Protein, Human

• Gene Name:

  Casein kinase 2, alpha 1 polypeptide

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  N-GST

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  The specific activity was determined to be 9 nmol/min/mg using casein as substrate.

• Form:

  Liquid

• Buffer:

  Supplied as sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM GSH, pH 8.0 Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins

• Molecular Weight:

  The recombinant human CSNK2A1/GST chimera consists of 616 amino acids and predicts a molecular mass of 71.4 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CSNK2A1

• Species:

  Human

• Protein ID:

  NP_808227.1

• Overview:

  A DNA sequence encoding the human CSNK2A1 isoform 1 (NP_808227.1) (Met 1-Gln 391) was fused with the GST tag at the N-terminus.

• AA Sequence:

  Met1-Gln391

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_808227.1