PCSK9 Protein, Human, Recombinant (His Tag), Biotinylated

• Background:

  Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

• Synonyms:

  FH3 Protein, Human; HCHOLA3 Protein, Human; LDLCQ1 Protein, Human; NARC-1 Protein, Human; NARC1 Protein, Human; PC9 Protein, Human

• Gene Name:

  Proprotein convertase subtilisin/kexin type 9

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Serine Proteases and Regulators

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins, Biological Target proteins, Biotinylated proteins

• Molecular Weight:

  The recombinant human PCSK9 consists of 673 amino acids and predicts a molecular mass of 72.5 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PCSK9

• Species:

  Human

• Protein ID:

  NP_777596.2

• Overview:

  A DNA sequence encoding the human PCSK9 (NP_777596.2) (Met1-Gln692) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.

• AA Sequence:

  Met1-Gln692

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_777596.2